Bioluminescence Resonance Energy Transfer (BRET) Service

Dorawest21 Jul, 2023Health

At present, there are many techniques for studying protein-protein interaction (PPI), each of which has its own advantages and disadvantages, and some methods for studying PPI have various limitations, especially in the study of dynamic PPI. A technology called bioluminescence resonance energy transfer (BRET) was developed about two decades ago. Since then, BRET has developed into a whole class of methods that can be used to investigate almost all types of PPI. It has high sensitivity and accuracy, and allows real-time monitoring of dynamic PPI in vivo, and uses easily measurable light as readout signal, which is very valuable for PPI function research. In addition, with the appropriate equipment, BRET can even be used as an imaging technique to map PPI localization.