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Lipid content analysis
Lipids are hydrophobic or amphipathic small molecules which include fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides and phospholipids. The important roles of lipids in biological physiology are evident not only in energy storage and structural components of cellular membranes, but also in signal transduction, membrane trafficking and morphogenesis. lipid content analysis is the synthesis and degradation of lipids in cells, consisting of the breakdown or storage of fats for energy and the synthesis of structural and functional lipids like those involved in the construction of cell membranes.
Inositol phosphate pathway
Inositol phosphates contain multiple phosphorylated derivatives and individual PIPs can contain different fatty acid chains, resulting in structural complexity. Creative Proteomics has built a dedicated analytical platform to provide you with qualitative and quantitative analysis of inositol phosphates. Based on ion chromatography separation techniques, we are able to accurately determine the total amount of phosphorylated derivatives. Based on LC/MS technology, we are able to quantify inositol phosphate pathway containing specific fatty acid chain structures.
Starch analysis method
Starch is a polymeric carbohydrate consisting of a large number of glucose units connected by glycosidic bonds. Similarly, sucrose is a kind of carbohydrate and composed of two monosaccharides: glucose and fructose. Basically, the starch and sucrose metabolism begin with D-fructose that interacts with a D-glucose in a reversible reaction through a maltodextrin glucosidase. By multiple procedures, the metabolism of starch and sucrose produce several important intermediates like glucose-6-phosphate and fructose-6-phosphate, which interact with other metabolic pathways including glycolysis and gluconeogenesis. Creative Proteomics has advanced mass spectrometry instrumentation and a team of bioinformatic analysts to provide starch analysis method.
Apoptosis and proinflammatory cytokines
Apoptosis plays an important role in the immune system, which is closely related to intracellular homeostasis, T cell aggregation, death of reactive lymphocytes and cytotoxic response of target cells. At present, it has been found that eukaryotic cell apoptosis mainly has the following pathways: 1. Cell receptor-mediated external apoptotic pathways; 2. Internal mitochondrial pathway; 3. B-granase mediated apoptosis pathway; 4. Endoplasmic reticulum stress pathway mediates apoptosis. The detection of cytokines related to apoptosis helps to study apoptosis and the physiological or pathological processes involved. Creative Proteomics can simultaneously quantitatively detect apoptosis and proinflammatory cytokines through a variety of technologies, and provide higher sensitivity and a wider detection range.
Brain inflammation cytokine
Inflammatory cytokines can regulate the response of pro-inflammatory cytokine production, mainly including IL-4, IL-5, IL-10, IL-13, transforming growth factor (TGF), epiodermal growth factor and so on. Among them, IL-4 can inhibit the production of other pro-inflammatory cytokines such as IL-1 and IL-6, as well as the production of lymphocytes and macrophages. IL-10 can inhibit the production of activated monocytes, macrophages, granulocytes and T cells. And IL-13 can inhibit the production of pro-inflammatory transmitters and down-regulate the function of monocytes with cytotoxicity. TGF-? can inhibit the inflammatory response of intestine. And EGF can stimulate tissue repair and protect the mucosa of gastrointestinal tract. Creative Proteomics can provide cytokine detection platform for brain inflammation cytokine research.
Serum cytokine analysis
Serum and plasma samples provide a holistic view of systemic responses. These liquid components of blood circulate throughout the entire body, offering a comprehensive representation of the intricate cytokine networks at play. This approach ensures that the analysis captures the full spectrum of cellular communication, enabling a more nuanced understanding of physiological processes. Creative Proteomics can provide you with a one-stop serum cytokine analysis for cytokine and protein detection and quantification.
Protein lipidation
Lipid modifications include Glycosyl-phosphatidylinositol anchor (GPI). At the C-terminus of proteins, it happens in the endoplasmic reticulum that the post-translational linkage between glycosylphosphatidylinositol and the modified proteins are translocated to the membrane, which are remain anchored to the outer leaflet of the cell membrane. Methods for detecting protein lipidation have been exploited to predict GPI-anchored proteins, N-myristoylated, S-isoprenylated or S-palmitoylated proteins, and so on. The traditional approaches are based on the incorporation of radioactive precursors and subsequent detection of the proteins by fluorography. Mass spectrometry (MS) methods are universally applied, which are for the mapping of post-translational modifications. This technology submits crucial advantages. Bio-orthogonal probes, reactions compatible with biomolecules that allow their study in their native settings.
C terminus of protein
The C-terminal sequence is an important structural and functional part of proteins and peptides, and plays a role in the biological function of proteins. In the analysis process of recombinant protein expression and purification products, especially the development and process establishment of recombinant protein products, the C-terminal sequence of the protein needs to be identified and analyzed to study the type of degradation products of protein drugs and the specificity of enzyme cleavage . Our protein C-terminal sequencing technology service can provide C-terminal sequencing analysis methods for biopharmaceutical proteins or other biological reagents such as MALDI-ISD for Top-down protein C-terminal sequencing and carboxypeptidase hydrolysis. Creative Proteomics is a reliable biopharmaceutical partner with professional proteomics research resources. We use first-class experimental platform and mature technical methods to provide you with high-quality one-stop service.
Circular dichroism spectroscopy
Circular dichroism (CD) spectroscopy is a fast, simple, and accurate method for studying protein structures in dilute solutions. The structural analysis was performed using the circular dichroism of proteins and the difference in absorption of left and right circularly polarized light by asymmetric molecules. As a reliable partner of biopharmaceutical companies, Creative Proteomics can provide customers with far UV circular dichroism spectroscopy analysis services based on GLP / GMP. The detectable molecules range from small chiral drugs and transition metal complexes to biological macromolecular structures such as proteins, glycoproteins and nucleic acids.
Pentose phosphate pathway
LC-MS/MS platform enable simultaneous relative and absolute quantification of multiple metabolites in a biological system. The results are widely used for biomarker discovery by investigating the relative changes in metabolite concentrations. The resulting data have been widely used to for biomarker discovery. By providing the exact molecular weights and retention time, LC-MS/MS techniques serves as a powerful analytical tool for identification and quantification of small molecules (metabolites). Creative Proteomics has established sensitive, reliable, and accurate HPLC-MS/MS method for quantification of metabolites in pentose phosphate pathway metabolites.
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