Fluorescence Resonance Energy Transfer (FRET) Service

Dorawest21 Jul, 2023Health

Nowadays, fluorescence resonance energy transfer (FRET) is increasingly used in biomedical research. FRET relies on the distance-dependent transfer of energy from donor molecules to acceptor molecules, so it is widely used to study molecular interactions. The donor molecule is the dye or chromophore that initially absorbs energy, and the acceptor is the chromophore that subsequently transfers energy. This resonance interaction is not converted into heat energy, nor is there any molecular collision. For FRET to occur, the following conditions must be met: 1. The donor and acceptor fluorophores must be close to each other; 2. The emission spectrum of the donor fluorophore must overlap with the absorption spectrum of the acceptor fluorophore; 3. The energy transfer from the donor fluorophore to the acceptor fluorophore occurs via intermolecular dipole-dipole coupling.