In the traditional biochemical analysis process, the contribution of the intracellular environment is usually lost. In order to study the interactions between molecules in cells, and to understand how cell architecture affects the formation of these complexes, live-cell imaging technology has developed rapidly, coupled with the use of genetically encoded fluorescent proteins (FPs), we have now achieved the tracking of assembly events such as protein complexes within the organized microenvironment of the living cell. We provide a variety of advanced imaging technologies, including Raman microscopy, fluorescence resonance energy transfer microscopy, and fluorescence lifetime imaging microscopy, etc. These tools provide an important supplement for biochemical research, extending the analysis of protein-protein interactions, protein conformational changes and signal transduction to their natural environment within the intact cell.
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