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Important Things To Know About Plasmid Preparation

Retrogeninc07 Apr, 2021News

The precipitation of the less supercoiled and large chromosomal DNA occurs with the addition of acetate-containing neutralization buffer. On the other hand, the small bacterial DNA plasmids remain in the solution only. So, there are many important advantages of this plasmid DNA preparation which you need to know about before opting for related services.

Diagnostic Testing for COVID 19 | Retrogen, Inc.

Retrogeninc02 Apr, 2021Technology

We are now offering COVID 19 testing at the Retrogen, Inc.! In order to ensure the safety of our staff and patients, we have developed the following testing protocol. The back door of our pharmacy will be the entrance for our COVID 19 testing site. One of our pharmacists will be prepared with protective equipment to screen for COVID using a nasal swab.

Sanger DNA Sequencing Steps and Method

Retrogeninc24 Mar, 2021Technology

This again helps in the identification of the base at the end portion of a specific fragment of DNA. Sanger performs this step by removal of an oxygen atom specifically from the ribonucleotide. This particular nucleotide is known as the dideoxynucleotide. So, this is how this process takes place. One important thing that you should know about is that both Sanger Sequencing Services and PCR make use of similar starting materials.

What Are Retrogen’s Covid-19 Test Benefits

Retrogeninc18 Mar, 2021Technology

Our molecular testing methods offer a better and advanced approach for in vitro diagnostic solution for real-time RT PCR detection of RNA from SARS-CoV-2. Retrogen uses TaqPath COVID-19 Combo kit FDA EUA qualitative nucleic acid real-time reverse transcription polymerase chain reaction (real time RT-PCR) assay. Our test utilizes direct real-time RT-PCR assay is intended for the use in detection of nucleic acid from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasal swabs (Anterior Nasal) specimens. Covid 19 testing assay is an aid in the diagnosis of SARS-CoV-2 infection.

What Is NGS Sequencing?

Retrogeninc11 Mar, 2021Technology

Not only that, but this is regarded as the gold standard sequencing technology. So, the results of NGS sequencing are mainly verified by using the Sanger sequencing. Also, the NGS enables the interrogation of multiple genes present in various samples all at one time. It also involves the analysis and discovery of diverse types of genomic features in a single sequencing run.

Working Methodology Of The Sanger Sequencing

Retrogeninc26 Feb, 2021News

In this step, also there will be the addition of complementary nucleotide from the template DNA strand. Again, for finding out the proper composition of the DNA sequence, it is important to bring this reaction to a defined stop. This again helps in the identification of the base at the end portion of a specific fragment of DNA. 16s Sequencing performs this step by removal of an oxygen atom specifically from the ribonucleotide. This particular nucleotide is known as the dideoxynucleotide.

Utilizing Gold-Standard Sanger Sequencing and Next-Gen Systems

Retrogeninc17 Feb, 2021News

NGS today is more than ever about how different organisms use genetic information and molecular biology to survive and reproduce with and without mutations, disease, and diversity within their population networks and changing environments. In this chapter, the advances, applications, and challenges of NGS are reviewed starting with a history of first-generation sequencing followed by the major NGS platforms, the bioinformatics issues confronting NGS data storage and analysis, and the impacts made in the fields of genetics, biology, agriculture, and medicine in the brave.

Bioinformatics & DNA Sequencing | Retrogen, Inc.

Retrogeninc03 Feb, 2021Health

The isolation of plasmid DNA from bacteria is a crucial technique in molecular biology and is an essential step in many procedures such as cloning, DNA sequencing, transfection, and gene therapy. These manipulations require the isolation of high purity plasmid DNA. The purified plasmid DNA can be used for immediate use in all molecular biology procedures such as digestion with restriction enzymes, cloning, PCR, transfection, in vitro translation, blotting and sequencing.

General Considerations for Plasmid DNA Preparation | Retrogen, Inc.

Retrogeninc29 Jan, 2021Business

Plasmid preparation from bacterial culture often involves two steps: lysis of the bacteria and purification of plasmid DNA. Alkaline lysis is the most commonly used method for cell lysis for small scale preparation. But multiple methods exist for the plasmid DNA sequencing purification step. These method have different advantages and disadvantages and may affect the downstream molecule biology applications.

DNA Sequencing: Clinical Applications Of New DNA Sequencing Technologies

Retrogeninc21 Jan, 2021Business

Availability of the reference human genome sequence enabled the generation of oligonucleotide probes and short sequence markers (SNPs) mapped to specific chromosome positions that could then be used to generate microarray platforms to analyze micro biome sequencing from specific patients to search for genomic copy number variants and genomic structural variants. Polymorphic SNP markers also facilitated analysis of segregation of specific genomic regions in families and analysis of inheritance of specific genomic regions.