General Considerations for Plasmid DNA Preparation | Retrogen, Inc.

Retrogeninc29 Jan, 2021Business

Plasmid preparation from bacterial culture often involves two steps: lysis of the bacteria and purification of plasmid DNA. Alkaline lysis is the most commonly used method for cell lysis for small scale preparation. But multiple methods exist for the plasmid DNA sequencing purification step. These method have different advantages and disadvantages and may affect the downstream molecule biology applications.