Moreover, sphingoid bases were shown to affect signaling pathways such as protein kinase C and phosphatidic acid. In addition, it has been suggested that increased Sphingoid bases increase with aging in yeast. Sphingoid bases were demonstrated to mediate the aging process by inhibiting mitochondrial fusion and causing fragmentation, resulting in decreases in mtDNA copy numbers, ATP levels, mitochondrial membrane potentials, and oxygen consumption. Creative Proteomics uses liquid chromatography mass spectrometry (LC-MS) method to identify the samples, which can effectively and accurately detect the changes in the content of sphingoid bases.
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