bioID

Dorawest22 Dec, 2023Health

BioID-MS excels in identifying weak, transient, insoluble, and post-translation modified protein interactions, potentially surpassing AP-MS in detecting low-abundance proteins. It has been applied in coronavirus replication, HIV protein interaction networks, bacterial host recognition, and mitosis-related protein interactions. However, low-expressing PPI partners may lead to false negatives, and biotinylation itself may impact protein behavior/interactions in certain cases.