Catecholamine hormones
Catecholamines and their metabolites are rather low in biological samples. This pose a great challenge to sensitivity and pretreatment of the detection methods. In immunoassays, cross-reactivity and non-specific binding may be a problem because they can lead to false-positive results. Thought HPLC-ECD is popular in the analysis of CA and their metabolites. HPLC-ECD is rather labor consuming and time-consuming because derivatization steps are essential in HPLC-ECD. The coeluting interference and electrode fouling compromise the reliability of HPLC-ECD. Creative Proteomics offers the HPLC-MS platform with excellent specificity and sensitivity.
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