What is siRNA and siRNA-seq

Kiko20 Jun, 2025Health

Small interfering RNA (siRNA) are short, non-coding RNA molecules, typically 20-30 nucleotides long, that play a crucial role in the post-transcriptional regulation of gene expression. siRNA was first discovered in plants and later identified in other organisms, including the model organism Caenorhabditis elegans and mammalian cells. Its discovery was pivotal, changing our understanding of gene regulation and setting the stage for new therapeutic approaches targeting specific genes.

How to Annotate Clusters in Seurat

Kiko03 Mar, 2025Health

Precise annotation of clusters in Seurat plays a critical role in extracting valuable insights from single-cell RNA sequencing (scRNA-seq) datasets. By associating computationally detected clusters with biological relevance, researchers can better understand cellular heterogeneity and functionality. This guide offers a comprehensive step-by-step overview of methods, tools, and strategies for effective cluster annotation, aiming to achieve reliable and high-quality outcomes in scRNA-seq analyses.

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