Tandem affinity purification

Dorawest31 Jul, 2024Health

Tandem affinity purification (TAP) is a rapid technique used to study protein-protein interactions in vivo. Through two steps of specific affinity purification, TAP allows for the rapid isolation of proteins that interact with the target protein under physiological conditions. Originally developed for use in yeast, the TAP method has rapidly evolved due to its versatility, efficiency, high purity, and low false positives. It has since been successfully applied to the study of protein interactions in various other organisms, including mammals and plants.