Plant hormones are highly bioactive signaling molecules that act as chemical messengers, mediating and controlling physiological processes during plant growth and development as well as stress responses by additive, synergistic, or antagonistic actions. Plant hormones are often present in trace amounts and their levels depend strongly on the plant organ, plant developmental stage, and environmental conditions. Plant hormones are structurally diverse active compounds that are divided into several major groups including auxins, cytokinins (CTK), gibberellins (GA), abscisic acid (ABA), ethylene (ETH), jasmonate (JA), salicylic acid (SA), strigolactone (SL) and brassinosteroids (BR). Mass spectrometry is a sensitive analytical technique that enables detection, identification and quantification of a variety of plant hormones by measuring their mass and characterizing their chemical structure, even at low abundance. MS instruments combine with either gas chromatography (GC) or liquid chromat
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