PiggyBac Transposition System

Creativebiolabs24 Oct, 2023Technology

PB transposon was originally identified from the genome of the cabbage looper moth, Trichoplusia ni, in the 1980s. Because of its high activity after being introduced into mammalian genomes, it has become an efficient gene transfer tool in genetic engineering research. PB transposon system is composed of three elements, a transposable element, a transposase, and a gene of interest. PB transposase can recognize inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector. It efficiently integrates the genes of interest between the two ITRs in the PB vector into target genomes. PB is initially used as gene delivery tool in insects. Recently, studies have reported that PB has the highest transposition rates and cargo capacities in mammalian cell lines and mouse germline cells.