Microfluidic chips have gradually become powerful tools for biological analysis in the fields of fundamental biology, clinical applications, food safety, and environmental monitoring due to their high sensitivity, high throughput, and low cost. Among them, the timely detection and analysis of biologically active small molecules such as oligonucleotides, oligopeptides, oligosaccharides, vitamins, minerals, and metabolites in physiological processes is very important for guiding therapeutic medication and predicting disease prognosis.
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