M6A sequencing

Kiko29 May, 2024Health

CD Genomics offers the transcriptome-wide analysis of the location of m6A in mRNA transcripts and other RNAs by methylated RNA immunoprecipitation sequencing (MeRIP-seq). MeRIP-seq uses an antibody that specifically detects and characterizes of N6-methyladenosine (m6A) in RNA. As 3'UTR of mRNA plays important role in mRNA stability, localization, and translation, and may affect the binding of RNA-regulatory proteins to these regions, enrichment of m6A in this region indicates that m6A may affect RNA metabolism and gene expression. Inhibition of the enzymes that involve in the RNA methylation has also provided clues to the potential biological roles of this modification. Hence, the identification and characterization of modified bases in RNA may lead to a better understanding of biological pathways.