Cells used in in vitro cell culture, especially animal cell culture, get attached to the surface of the container. These cells need to be detached for further use. Detachment of adherent cells from the surface or substrate to which these are attached is done with the help of cell dissociation reagents. Cell dissociation reagents along with chelators are largely used during cell passaging. The cell dissociation reagents carry out collagenolytic and proteolytic activity for the disengagement of cell lines and tissues from the plastic or glass surfaces to increase the isolation efficiency with high cell viability.