What is HPLC Column? A Complete Guide to Types, Uses, and Chemistry
What is HPLC Column? An HPLC column is a precision-engineered cylindrical tube packed with stationary phase material that allows for the separation of compounds in a sample based on their chemical interactions. It works by retaining analytes differently depending on their molecular characteristics and their interaction with both the stationary phase and the mobile phase. The column is housed in a durable stainless steel shell, capable of withstanding the high pressures involved in liquid chromatography. It connects directly to the HPLC system and determines critical performance factors like resolution, selectivity, and sensitivity.
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