Phi29 DNA Polymerase

Ivacolter24 Nov, 2023Health

Phi29 DNA Polymerase is a 74.4 kDa purified recombinant protein inducibly expressed in E. coli carrying the ?29 DNA polymerase gene. With high sensitivity and great synthesis ability, it can be used for whole genome amplification from a single cell. It possesses 3'?5' proofreading exonuclease activity to ensure high fidelity of resulting DNA. Its properties of multiple strand displacement and processive synthesis allow >10 kb lengths of amplification products from genome or plasmid DNA, and enable rolling-circle replication of circular DNA.