The 96 well plate cell seeding density of cells in culture significantly affects cell behavior, growth, and response to experimental conditions. Too low a density may result in insufficient cell-cell interactions and reduced viability, leading to unreliable data. On the other hand, excessively high densities can lead to competition for nutrients and space, altering cellular physiology and complicating data interpretation. Achieving an optimal seeding density is crucial for obtaining reliable and reproducible results
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