On the other hand, the counteranion remains almost completely in the bulk solution; and the concentration increases with each cycle. A clever way to overcome it is to take advantage of its cumulative nature: it uses nitrate as a counter anion, i.e. iron nitrate (Fe(NO3)3) as a coagulant. In this way, the remaining nitrate ions after each harvest and medium reuse cycle can be used as a nitrogen source for the next round of cultivation. In this study, this hypothesis was tested and proven. In this study, Chlorella sorokiniana was successfully cultured in circulating medium with nitrogen supplied directly by the coagulant Fe(NO3)3.
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