Escherichia coli Lipopolysaccharides
LPS is dispersable in aqueous solvents at concentrations of 1mg/ml. To achieve suspension in water, heating to about 50°C with intermittent vortexing or sonication is generally recommended. Allow ample time for dispersion to occur. The use of 0.5% triethylamine aids in dispersion. Triethylaine is very basic and may be neutralized with Tris HCl to avoid hydrolysis of the fatty acid chains.
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