Dengue virus research should be conducted using biosafety level 2 practices, equipment, and facility design. Laboratory diagnostic methods to confirm dengue virus infection may involve detection of virus, viral nucleic acid, antigen or antibody, or a combination of these techniques. The virus can be detected in serum, plasma, circulating blood cells, and other tissues for 4-5 days after onset. In the early stages of the disease, viral isolation, nucleic acid or antigen testing can be used to diagnose infection. At the end of the acute phase of infection, serology is the method of choice for diagnosis. Serological tests for dengue virus generally use IgM antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA), IgG ELISA, IgM capture and IgG capture ELISAs, anti-dengue virus IgA capture ELISA, and haemagglutination-inhibition (HI) test.
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